Alpha Psi Omega, Wilmington, NC
Chi Iota Omega, Kenansville, NC
Chi Omega, Rocky Mount, NC
Delta Alpha, Fayetteville State University
Epsilon Phi Omega, Goldsboro, NC
Gamma Beta Omega, Wilson, NC
Iota Kappa Omega, Greenville, NC
Lambda Delta Omega, Williamston, NC
Omicron Lambda, UNC Pembroke
Omicron Phi, UNC Wilmington
Phi Theta Omega, Washington, NC
Rho Alpha Omega, Laurinburg, NC
Rho Omega Omega, Clinton, NC
Sigma Iota Omega, Fairmont, NC
Tau Gamma, NC Wesleyan College
Tau Omega Omega, Jacksonville, NC
Theta Alpha, East Carolina University
Theta Beta Omega, New Bern, NC
Upsilon Kappa Omega, Fayetteville, NC
Zeta Omicron Omega, Kinston, NC
Zeta Pi Omega, Fayetteville, NC

Northern Carolina Cluster (31 Chapters)
Alpha Chi, North Carolina Central University
Alpha Phi Omega, Danville, VA
Alpha Phi, NC A&T State University
Alpha Theta Omega, Raleigh, NC
Alpha Zeta Omega, Durham, NC
Beta Iota Omega, Greensboro, NC
Beta Rho, Shaw University
Chi Rho Omega, Knightdale, NC
Eta Omicron Omega, High Point, NC
Gamma Lambda, Winston-Salem State University
Gamma Xi, Saint Augustine's College
Iota Mu, Duke University
Kappa Delta Omega, Martinsville, VA
Kappa Omicron, North Carolina State University
Mu Omicron Omega, Chapel Hill, NC
Nu Chi Omega, Oxford, NC
Nu Rho, UNC Greensboro
Omicron Epsilon, Elon University
Omicron Omega Omega, Southern Pines, NC 
Omicron Psi Omega, Lexington, NC
Phi Omega, Winston-Salem, NC
Pi Beta, Wake Forest University
Pi Omicron Omega, Burlington, NC
Psi Gamma Omega, Hillsborough, NC 
Rho Tau Omega, Warrenton, NC
Sigma Kappa Omega, Greensboro, NC
Sigma Mu, High Point University
Sigma Nu Omega, Roxboro, NC
Sigma Tau Omega, Cary, NC
Theta Pi, UNC Chapel Hill
Zeta Xi, Bennett College

Alpha Epsilon, Virginia State University
Alpha Eta, Virginia Union University
Beta Chi Omega, Roanoke, VA
Chi Beta Omega, Falls Church, VA
Delta Omega, Petersburg, VA
Eta Phi Omega, Charlottesville, VA
Gamma Iota Omega, Lynchburg, VA
Kappa Rho Omega, Farmville, VA
Lambda Chi, James Madison University
Lambda Delta, Longwood College
Lambda Kappa Omega, Fairfax, VA
Omicron Chi Omega, Woodbridge, VA
Omicron Iota, George Mason University
Omicron Sigma, Lynchburg College
Phi Nu Omega, Alexandria, VA
Pi Rho Omega, Chesterfield, VA
Rho Eta Omega, Richmond, VA
Rho Mu, University of Richmond
Sigma Alpha, Strayer & Marymount Universities
Sigma Gamma Omega, Harrisonburg, VA
Tau Delta, Randolph-Macon College
Tau Mu Omega, Christiansburg, VA
Tau Phi Omega, Richmond, VA
Tau Zeta, Washington & Lee University
Theta Chi, Radford University
Theta Kappa, University of Virginia
Theta Phi, Virginia Polytechnic Institute (VA Tech)
Theta Rho, Virginia Commonwealth University
Upsilon Omega, Richmond, VA
Xi Upsilon Omega, Fredericksburg, VA
Zeta Chi Omega, Arlington, VA

Tidewater Cluster (25 Chapters)
Alpha Upsilon, St Paul's College
Delta Epsilon, Norfolk State University
Delta Theta, Elizabeth City State University
Gamma Delta Omega, Portsmouth, VA
Gamma Lambda Omega, Lawrenceville, VA
Gamma Theta, Hampton University
Gamma Upsilon Omega, Hampton, VA
Iota Omega, Norfolk, VA
Kappa Gamma, Old Dominion University
Kappa Iota Omega, Garysburg, NC
Lambda Gamma Omega, Virginia Beach, VA
Lambda Lambda Omega, Williamsburg, VA
Lambda Omega, Newport News, VA
Lambda Psi Omega, Franklin, VA
Nu Chi, College Of William & Mary
Nu Epsilon, Christopher Newport University
Nu Eta Omega, Edenton, NC
Omicron Pi Omega, Chesapeake, VA
Phi Chi Omega, Smithfield, VA
Pi Epsilon Omega, Exmore, VA
Sigma Omicron, Virginia Wesleyan College
Upsilon Omicron Omega, Norfolk, VA
Xi Rho Omega, Ahoskie, NC
Zeta Epsilon Omega, Suffolk, VA
Zeta Kappa Omega, Elizabeth City, NC

Western Carolina Cluster (15 Chapters)
Alpha Lambda Omega, Charlotte, NC
Alpha Xi, Livingstone College
Delta Psi, Barber-Scotia College (inactive)
Delta Xi Omega, Salisbury, NC
Gamma Delta, Johnson C. Smith University
Gamma Gamma Omega, Asheville, NC
Kappa Alpha, Western Carolina University
Kappa Kappa, UNC Charlotte
Lambda Upsilon Omega, Concord, NC
Omicron Kappa, Appalachian State University
Rho Psi Omega, Charlotte, NC
Sigma Psi, Davidson College
Tau Beta, Queens College
Xi Phi Omega, Hickory, NC
Zeta Mu Omega, Gastonia, NC